Additional file 2. A Measurement of human A levels (pg/mL) in Tg and TgMT5-/- cultures using the ThermoFisher Scientific ELISA kit. B and C. mRNA levels of hAPP and hPSEN1 analyzed by RT-qPCR in Tg and TgMT5-/- cultures and normalized with Gapdh as housekeeping gene. Black bars represent control (untreated) conditions and grey bars IL-1β treated conditions (10 ng/mL for 24 h). Values are the mean ± SEM of 3–5 independent cultures by genotype.