Figure 2: Ungulate species tree constructed with NOD1 coding sequences shows relationships proportional to differences in gene sequences. To construct these trees, NCBI Taxonomy browser was used to collect ungulate phylogenetic relationship in Newick standard format. The scientific names denoted in the species table were used to collect taxonomic information. Using the MUSCLE aligned NOD1 gene sequences, the UPGMA (unweighted pair group method with arithmetic mean) was employed to compare the number of changes between all sequences, and clusters sequences (or groups of sequences) with the fewest possible differences. This generated another Newick standard file that could then be piped into Figtree. This figure shows the relationship between different ungulates based on NOD1 sequence similarity compared to their entire genomic relationship.