Supplemental Figure 2. Extracellular vesicles (EVs) from SW480 IMP1Null cells isolated by size-exclusion chromatography (SEC). Fractions (fx) 3-15 are shown. For Fx 3-11, 70µL of sample was concentrated by boiling and loaded on the gel. Protein concentration was below detection for these samples. For Fx 12-15, 15µg of protein were loaded. HT-29 IMP1OE whole cell lysate (WCL) was used as the loading control. Western blotting was used to detect tetraspanin protein and EV marker, CD9 and determine which SEC fractions to collect for subsequent experiments.