High quality time lapse of GFP labelled B. subtilis 3610 (NRS1473, green) exhibiting crowd movement around a lettuce root (Lactuca sativa, red) growing in sulphorhodamine B stained transparent soil (magenta). Images were captured in 60-minute intervals using a 10X objective. The time lapse covers a 24 hour period, approximately 24 hour post patch inoculation of the B. subtilis. The lettuce seedling is 3 days old.