Additional file 3: Fig S3. Catalytic P300/CBP inhibitors perturb cellular transcription. (A) RNA-seq AT3 cells treated for 3 h with A-485 (1 µM) or A-241 (250 nM) relative to DMSO. (B) Venn diagram overlap of differentially expressed genes (P-value < 0.01) following treatment of AT3 cells with A-485 or A-241 relative to DMSO, respectively. (B) Row-normalized heatmap of gene expression values from RNA-seq of AT3 cells treated with A-485, A-241, or DMSO vehicle for genes responsive to A-241 treatment (P-value < 0.01). (D) H3K27ac ChIP-Rx signal (normalized log2 counts per million) in presence of A-241 or DMSO for 3 h at cis-regulatory elements active under baseline conditions (in the absence of IFNγ) as identified by ATAC-seq. (E) Log2 fold-change in H3K27ac ChIP-Rx at active cis-regulatory elements across 10 quantiles ranked by loss of H3K27ac. (F) Log2 fold-change in ATAC-seq signal at active cis-regulatory elements across 10 quantiles ranked by loss of H3K27ac. ****p < 0.0001, Mann–Whitney U test.