Additional file 3: Figure S3. Characterization of iPSC-derived NPCs from two other iPSC clonal lines. A: Gene expression of pluripotency marker (NANOG and OCT4) and NPC marker (PAX6, SOX1, CXCR4) in NPCs from iPSC clonal lines 2 (upper row) and 3 (lower row) over the course of 15 passages, measured by qPCR. B: Flow cytometry analysis of iPSCs (upper row) and NPCs from iPSC clonal lines 2 and 3 at different passages for Oct4, Pax6 and Sox1. Pie charts illustrate percentage positivity (light/dark blue) for the respective marker and cell type. n.t.: not tested.