DNA assembly pertains to the annealing of different DNA fragments in the correct order and orientation to form a fully assembled plasmid or expression vector. This process is typically carried out in manual steps at the scale of a microcentrifuge tube. Recent work in automating genetic engineering has explored the use of microfluidic chips for automating more aspects of the plasmid assembly process. This set of slides presents a proposed design for a DNA assembly chip. Specifically, each input port helps channel one gene fragment, so the number of input ports will theoretically scale with the number of gene fragments. The gene fragments will be manually pipetted in, and with fluidic pressure, will be led to a central mixing chamber where a DNA ligase will ligate the annealed DNA plasmid (through sticky ends) into a full covalent linked plasmid. Overall, it is hoped that the concepts articulated in this proposed design will spur more innovations in chip-based DNA assembly.