These are tiled x-ray images of eight cockroach species taken at Argonne National Labs. The species are: Blaberus discoidalis, Paratemnopteryx couloniana, Deropeltis paulinoi, Dorylaea orini, Panchlora nivea, Gyna lurida, Elliptorhina chopardi, and Gromphaedorhina portentosa.
All species were taken to Argonne National Laboratory (Argonne, IL, USA) for phase contrast x-ray synchrotron imaging . The animals were euthanized using ethyl acetate prior to placement in the field of the x-ray beam and then placed inside a small Kapton (x-ray transparent) pouch before imaging. Specimens were mounted individually on a translation/rotation stage and imaged with monochromatic x-rays of 25 keV. The x-rays were converted to visible light using a cerium-doped yttrium-garnet scintillator, located 300 mm from the sample. A CCD camera (SensiCam QE, Cooke, Romulus, MI, USA) and 5X microscope objective (Achrovid, Infinity, Boulder, CO) were used to record the images (1280 X 1024 pixels).
Using Adobe ImageReady CS2 a full composite image for each animal was generated by tiling the images, maintaining the same resolution throughout the process. The number of tiles per animal ranged from approximately 100 in the smallest specimens to 3000 in the largest specimens. Each tile has an overlap of 445 pixels with the adjacent tiles on the left and right, and an overlap of 190 pixels with adjacent tiles above and below. The correct overlap was found by manually overlapping and matching easily distinguishable features in Adobe ImageReady CS2 and then measuring tile overlap. Adobe ImageReady CS2 allowed us to arrange the tiles in rows with the correct overlap by inputting "-445" in the "distribute layer horizontal space" function field. Tiles in these rows were then merged. Merged rows were arranged with the correct overlap by inputting "-190" in the "distribute layer vertical space" function field. All rows were then merged forming the full composite image. Full composite images were then saved as .PSD Photoshop Documents and opened in Adobe Photoshop CS4. Full composite images were uniformly contrast adjusted to provide better clarity of tracheae. Completed images were saved with the .TIFF file format in Adobe Photoshop CS4 to be imported into ImageJ ((rsbweb.nih.gov/ij/).
Before posting to FigShare, images were cropped and reduced in size to 100 inches wide in order to reduce file sizes. Higher resolution images are available upon request. ...
All species were taken to Argonne National Laboratory (Argonne, IL, USA) for phase contrast x-ray synchrotron imaging . The animals were euthanized using ethyl acetate prior to placement in the field of the x-ray beam and then placed inside a small Kapton (x-ray transparent) pouch before imaging. Specimens were mounted individually on a translation/rotation stage and imaged with monochromatic x-rays of 25 keV. The x-rays were converted to visible light using a cerium-doped yttrium-garnet scintillator, located 300 mm from the sample. A CCD camera (SensiCam QE, Cooke, Romulus, MI, USA) and 5X microscope objective (Achrovid, Infinity, Boulder, CO) were used to record the images (1280 X 1024 pixels).
Using Adobe ImageReady CS2 a full composite image for each animal was generated by tiling the images, maintaining the same resolution throughout the process. The number of tiles per animal ranged from approximately 100 in the smallest specimens to 3000 in the largest specimens. Each tile has an overlap of 445 pixels with the adjacent tiles on the left and right, and an overlap of 190 pixels with adjacent tiles above and below. The correct overlap was found by manually overlapping and matching easily distinguishable features in Adobe ImageReady CS2 and then measuring tile overlap. Adobe ImageReady CS2 allowed us to arrange the tiles in rows with the correct overlap by inputting "-445" in the "distribute layer horizontal space" function field. Tiles in these rows were then merged. Merged rows were arranged with the correct overlap by inputting "-190" in the "distribute layer vertical space" function field. All rows were then merged forming the full composite image. Full composite images were then saved as .PSD Photoshop Documents and opened in Adobe Photoshop CS4. Full composite images were uniformly contrast adjusted to provide better clarity of tracheae. Completed images were saved with the .TIFF file format in Adobe Photoshop CS4 to be imported into ImageJ ((rsbweb.nih.gov/ij/).
Before posting to FigShare, images were cropped and reduced in size to 100 inches wide in order to reduce file sizes. Higher resolution images are available upon request. ...