These supplementary videos provide examples of larva, pupa, and adult hearts beating over time with segmented masks. The left side provides the segmented ground truth overlayed onto the optical coherence microcscopy video, the ground truth mask by itself (red), the FlyNet 2.0+ mask (blue) and the overlap between the two (purple). The right side shows IOU (top), area (middle), and heart rate (bottom) over time.