Rice is a staple food and one of the world’s major grain crops, serving as the staple food for 2/3 of the world population. Rice is rich in bioactive compounds such as γ-Oryzanol (GO), remarkably predominant in the bran fraction (Pereira et al, 2021). Previous studies have explored the physiological effects of GO, including as antioxidant, antidiabetic, anti-carcinogenic, neuroprotective, and hypocholesteremic effects (Rungratanawanich et al., 2020). GO is a mixture of ferulic acid (FA) esters of triterpene alcohols and plant sterols present in cereals such as rice. The GO composition is characterized by several isomers that determine its final concentration and profile in the rice grain. About ten GO molecular species have been reported to date (Miller & Engel, 2006; Kim et al., 2013). The major components of rice GO are cycloartenyl ferulate (CAF), 24-methylenecycloartanyl ferulate (24MCAF), campesteryl ferulate (CampF), and β-sitosteryl ferulate (SF) that account for more than 80% of GO in rice bran. The aim of this work was to identify the major components of GO in whole flour and bran of 5 different rice varieties (Arelate, Ariete, Caravela, Carnaroli and Giza 177) approached in the TRACE-RICE project, as well as other minor components including 7-campestenyl ferulate, campestanyl ferulate (CampF), cycloartanyl ferulate, stigmasteryl ferulate (SF), sitostenyl ferulate and sitostanyl ferulate, using Liquid chromatography associated to different mass spectrometry-based approaches, namely the high-resolution mass spectrometer Orbitrap and QTRAP LC-MS/MS. The QTRAP mass spectrometer enabled to identify the four major and six minor GO components. However, because some of these minor components share the same precursor to product ion transitions as some of the major components, resolution is hindered. For example, the resolution of the major component CampF is affected by the presence of the minor components 7-campestenyl ferulate and campestanyl ferulate and the same for SF and sitostanyl ferulate. Using the orbitrap mass analyzer the four major GO components were identified in rice samples and only one minor component (cycloartanyl ferulate).