1 Citation
Additional file 4: Fig. S4. Combined treatment induces differential metabolic reprogramming. a. Intracellular metabolites profile in MCF-10A and MDA-MB-231 treated with DXR or STS+DXR. Cells were harvested on day 0, day 1 and day 2. *P ≤ 0.05. b. Expression of ATP5A in triple negative cell lines MDA-MB-468 and HS578 upon DXR or STS+DXR assessed by immunofluorescence analysis. Left panel: representative micrograph where ATP5A is shown in green, nuclei were counterstained with DAPI (blue). Scale bar, 20 μm. Right panel: Quantification, where data are presented as mean corrected total cell fluorescence (CTCF) ±SD. *P ≤ 0.05. c. Expression of ATP5A in MCF-7 and SKBR-3 cell lines upon DXR or STS+DXR assessed by immunofluorescence analysis. Left panel: representative micrograph where ATP5A is shown in green, nuclei were counterstained with DAPI (blue). Scale bar, 20 μm. Right panel: Quantification, where data are presented as mean corrected total cell fluorescence (CTCF) ±SD. *P ≤ 0.05. #Statistical trend.