1 Citation
Additional file 5: Fig. S5. ATP5A1 silencing mimicks the effect of combined STS+DXR treatment in TNBC cells - FOXO1 silencing eliminate the selective sensitivity of TNBC cells to STS+DXR treatment. a. Transcriptional levels of ATP5A1 upon ATP5A1 silencing in MCF10A and MDA-MB-231. Data are presented as mean expression values. *P ≤ 0.05. b. Transcriptional levels of FOXO1 upon FOXO1 silencing in MCF10A and MDA-MB-231. Data are presented as mean expression values. *P ≤ 0.05. c. γH2AΧ immunofluorescence staining foci per cell in MCF-10A and MDA-MB-231 cells with or without ATP5A1 silencing. Nuclei counterstained with DAPI. Scale bar, 5μm. Data are presented as mean of the number of foci per cell. *P ≤ 0.05. d. γH2AΧ immunofluorescence staining foci per cell in STS+DXR treated MCF-10A and MDA-MB-231 cells with or without FOXO1 silencing. Nuclei counterstained with DAPI. Scale bar, 5μm. Data are presented as mean of the number of foci per cell. *P ≤ 0.05. e. Transcriptional analysis of NRF2 coupled with the downstream targets NQO1 and TXNRD1 in MCF-10A and MDA-MB-231 cell lines with or without ATP5A1 silencing. Data are presented as mean expression values. *P ≤ 0.05. f. Transcriptional analysis of NRF2 coupled with the downstream targets NQO1 and TXNRD1 in STS+DXR treated MCF-10A and MDA-MB-231 cell lines with or without FOXO1 silencing. Data are presented as mean expression values. *P ≤ 0.05.