Additional file 1: Supplementary figure 1. (A) Expression of CD15 and CD14 on low-density granulocytes (LDG) from two pregnant women with SLE (two left panels, samples from trimester three) and two healthy pregnant controls (HC, two right panels, one sample from trimester three and one sample from trimester one). (B) Analysis of proportions of LDG and shedding of CD62L by NDG in blood from pregnant women with SLE and healthy pregnant controls between 17 and 24 h post venipuncture i.e., the time span when all samples in the study were analyzed. (C) Analysis of total number of granulocytes, proportions of LDG and shedding of CD62L by normal-density granulocytes (NDG) from one pregnant woman with SLE in trimester three and one healthy pregnant control in trimester one at 5 and 24 h post venipuncture. Supplementary figure 2. Comparison of (A) proportions of low-density granulocytes (LDG), (B) proportions of LDG that have shed CD62L, (C) proportions of normal-density granulocytes (NDG) that have shed CD62L and (D) total granulocyte counts during compared to after pregnancy among women with SLE from whom late postpartum samples were collected. *p < 0.05, **p < 0.01 and ***p < 0.001, Kruskal-Wallis followed by Dunn’s multiple comparison test. Supplementary figure 3. Comparison of LDG proportions in pregnant women with SLE with or without a moderate/high disease activity (SLEDAI-2K ≥ 4) in (A) trimester one, (B) trimester two and (C) trimester three. Mann-Whitney U test. Supplementary figure 4. Comparison of (A) LDG proportions, (B) LDG activation by CD62L shedding, (C) NDG activation by CD62L shedding and (D) IFNα protein levels in SLE and healthy pregnancy for each trimester. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 Mann-Whitney U test. Supplementary figure 5. Comparison of the proportions of low-density granulocytes (LDG) and normal-density granulocytes (NDG) that have shed CD62L in pregnant women with SLE and in healthy pregnant controls (HC). **** p < 0.0001, Mann-Whitney U test. Supplementary figure 6. Concentrations of G-CSF (A) and GM-CSF (B) in plasma during pregnancy (first, second and third trimester) compared to the late postpartum period among women with SLE from whom late postpartum samples were collected. Concentrations of G-CSF (C) and GM-CSF (D) in plasma in the first, second and third trimester in SLE compared to healthy pregnancies. Supplementary figure 7. (A) OPLS loading column plot depicting granulocyte-r...