Additional file 8: Fig. S1. dCas9-TET1 modulates the epigenetic status of a conserved cancer-associated DMR. A Boxplot illustrating the DNA methylation levels of the indicated significant CpG sites located within the cancer-associated DMRs identified in CRC as determined by the 450 K array platform. Samples are coloured according to their dataset of origin, as indicated in Fig. 1A. B Dot plot depicting the DNA methylation status of the CpGs included in the above-mentioned DMR in the context of different dCas9-mediated DNA demethylation or transcriptional reactivation strategies, as determined by a cloning-based bisulphite sequencing protocol. Each line represents a different clone and the genomic coordinates of the different CpG sites contained in this region are indicated at the top. Black circles represent a hypermethylated CpG, while white circles indicate a DNA hypomethylation event. (TIF 2031 KB)