Additional file 13: Fig. S4. Activation of RSPO2 expression by transcriptional mechanisms also impairs the proliferation rate of DLD1 and HCT116 cells. A Expression levels of chimeric dCas9-VP64 and β-Tubulin proteins obtained by western blot analyses in control transduced or Cas9 transduced DLD1 and HCT116 cells. The approximate size of the protein products is indicated. B Barplots showing RSPO2 gene expression levels observed upon epigenetic modulation of its promoter region in DLD1 (top) and HCT116 (bottom) cells in the context of dCas9-VP64-related chimeras, both in control and RSPO2 targeting RNA conditions. C Boxplots representing the normalized cell proliferation rate observed for the indicated gRNA treatments at two different time points (24 and 96 h) in the context of DLD1 and HCT116 cells transduced with dCas9-VP64 constructs. For B, data represent mean ± standard deviation of at least 3 independent experiments, while for C, at least 8 experimental replicas were included. Two-sided Welch’s t tests were applied for the different statistical comparisons versus each corresponding control condition. ***p value < 0.001; **p value < 0.01; *p value < 0.05; n.s.—nonsignificant.