Additional file 3: Figure S1. Culture and identification of hiPSCs and hiPSC-CMs. (A) Flow chart of hiPSC differentiation into hiPSC-CMs. (B) Immunofluorescence identification of hiPSCs expressing NANOG, SOX2, and OCT4 stemness markers in hiPSCs. (C) Immunofluorescence identification of hiPSC-CMs expressing α-actinin, TNNT2, and CX43 myocardial markers. (D) Ultrastructure of hiPSCs and hiPSC-CMs. Mitochondria (red arrows), Z-band (blue arrows), sarcomere (yellow arrows).