Additional file 1: Fig. S1. Representative figures of neuronal loss stage in the amygdala. A–C Stage 0. Neuronal loss and glial proliferation is absent. D–F Stage 1. Mild neuronal loss with minimal gliosis is noted. G–I Stage 2. Moderate neuronal loss with gliosis is present, but tissue rarefaction is not evident. J–L Stage 3. Severe neuronal loss with remarkable glial proliferation is noted. Tissue rarefaction is also seen. Scale bars: A, D, G, J 200 μm, B, E, H, K 100 μm, C, F, I, L 50 μm.