Spatial ecology is the study of how landscape characteristics influence the distribution and movement of organisms within their environment. The Spatial Ecology Gateway (SEG) enables researchers, students and wildlife managers to upload biotelemetry data, typically GPS readings, and construct home ranges that allow them to interpret animal space use. Applications of the SEG can include classroom projects, basic research into problems in wildlife ecology, environmental impact studies and mitigation of adverse outcomes such as habitat fragmentation or increased human-wildlife interaction resulting from new development. The SEG insulates users from the underlying computational details so that they can focus on their science rather than mastering the technology. Users of the SEG have the option to generate two-dimensional (2D) or, where applicable, three-dimensional (3D) home ranges. Users are also provided with less computationally intensive tools to perform exploratory analyses.