Additional file 2: Figure S2. Creation of the 74bbz mutant clones. A GFP+ cells of the 74bbz mutants and parent CAR expressing Jurkat cells were sorted at the same intensity by flow cytometry. B An immunoblot of CD3ζ to show the expressing of parent, 543, 5311, 42105-74bbz clones. Endogenous CD3ζ was detected at 15 kDa while the chimeric CD3ζ on CAR was detected at 55 kDa. C CD74-ECD-Fc fusion protein was stained by Coomassie blue staining.