Figure S1: Validation of the results obtained from RNAseq data by RT-qPCR quantification on 10 additional samples (cancerous: CAN7-11, healthy: EDU7-11). A) Normalised Ct values (mean of three replicates) of 17 selected genes with contrasting expression levels and oncogenic relevance. Normalisation was performed on the mean of the Ct of three constitutively expressed genes (GAPDH, RPS28 and UBE2D3) within each sample; B) Comparison of Log2FC values between the two quantification methods (RT-qPCR and RNA-seq): Log2FCs for RNA-seq data have been calculated with DESeq2 on 9 samples (CAN1-3, EDU1-3, TRO1-3) and Log2FCs for RT-qPCRs have been calculated as follow for the 10 additional individuals (CAN7-11, EDU7-11): FC= efficiency of the qPCR mean of Ct of the 5 MtrBTN2-affected individuals - mean of Ct of the 5 MtrBTN2-free individuals