Additional file 1: Figure S1. Patients profile of clinicopathological data. 162 GC patients received with CTC examination. 57 patients received chemotherapy or abandoned due to advanced disease and poor physical condition, 105 patients received with radical surgery (total or subtotal gastrectomy with D2 lymph node dissection) and were classified as pT1Nx, pT2Nx, pT3Nx and pT4Nx stages according to NCCN guideline. The maximum DFS time was 18 month and the follow up of patients excluded with pT1Nx stage. The corresponding paraffined sections of patients were collected to perform immunohistochemical staining to detect RORα, G6PD and PFKFB3 expression levels. GC, gastric cancer; CTC, circulating tumor cells, pTNM, pathology Tumor-Node-Metastasis. NCCN, national comprehensive cancer network. DFS, disease free survival.