Fluorescence confocal images of lineage-depleted iWAT SVF from Bbs8+/+ and Bbs8-/- mice, labeled against Smoothened (green, SMO) ARL13B (red, cilia), g-Tubulin (magenta, basal body), and with DAPI (blue). Cells were treated with H2O (control) or 1 µM SAG for 24 h. Scale bars are indicated. In all images, the green channel (SMO) was shifted by 10 px to the bottom for better visualizing SMO accumulation in cilia.