This video captures the dynamic membrane fluctuations of a human erythrocyte treated with escin, a saponin commonly utilized in the treatment of chronic venous insufficiency, under phase contrast microscopy conditions. The footage was originally recorded at an acquisition rate of 250 frames per second (fps). With a spatial resolution of 0.05 microns per pixel, the video provides a high-resolution view of the erythrocyte's morphology and the subtle movements of its membrane.Such high-speed videography is crucial for studying the biophysical properties of cell membranes and can provide insights into erythrocyte flexibility, membrane stability, and cell health. This video can be particularly useful for researchers focusing on hematology, cellular biology, and biophysics, gaining insights into erythrocyte flexibility, membrane stability, and overall cell health, offering a unique perspective on how escin influences erythrocyte dynamics.This video is being shared and made publicly available as supplementary information for a scientific article that investigates the adaptation of the red blood cell to an external membrane stiffener. It is particularly beneficial for researchers focusing on hematology, cellular biology, and biophysics, providing a valuable reference for studies investigating the effects of therapeutic agents on red blood cell physiology or exploring membrane-related pathologies.We invite viewers to explore the associated article for a comprehensive understanding of the context and implications of these observations. This resource is intended to enrich the scientific discussion on red blood cell adaptability and membrane dynamics, and we welcome collaborations that aim to delve deeper into these fascinating cellular behaviors."