Supplemental Movie 3. Timeseries of data sub-volume shown in Figure 5.
SCAPE microscopy of a 3D cell culture composed of U87 glioblastoma cells stained with CellTracker Red for whole cell labeling and Oregon Green BAPTA-1 AM, a calcium sensitive fluorescent dyes as a functional reporter cellular Ca2+ dynamics. Video shows a zoomed region of the sample with maximum projections from 2 views. Right side shows time-courses of ratiometric calcium signals in the numbered cells indicated on the left panels. Data was acquired at one volume per 12.5 seconds for 30 minutes. The sample was irradiated with protons at around 10 minutes.