Figure 3.4A: Multiple sequence alignment of type II metacaspases extracted from the type II connected component in Figure 3.3. p20-like region and first part of the linker domain. The MSA was visualised in Jalview with Clustal colour scheme. The left-hand and right-hand regions of the alignment have been trimmed. The sequences are selected from regions A, B and C of the SSN. The orange box highlights a region of negatively-charged residues absent in region C sequences, while green boxes indicate regions of the linker domain absent here, but present in region B Streptophyta type II metacaspases. These features suggest they are likely homologues of AtMCAII-f. The catalytic His and Cys residues are marked with red asterisks.