Additional file 1: Figure S1. (A) MTT assay of THP-1 macrophages grown in RPMI medium 11mM glucose or in EV-depleted medium, for 24h. (B) Quantity of contaminant particles remained in EV-depleted medium detected by Microfluidic Resistive Pulse Sensing (MRPS) as in [28, 29]. (C) WB of the EV-depleted medium or the RPMI medium to detect APOB. (D) MTT assay of THP-1 macrophages grown in RPMI, or in RPMI 15 mM or 30 mM glucose. Values are expressed as % of untreated cells. (E) and (F), ELISA quantification of cytokines in the conditioned medium of macrophages.(G) WB protein quantification of the polarization markers CD163 (M2) and CD86 (M1) in macrophages treated either with mannitol 30mM (osmolarity control) or with glucose 30mM. Values are normalized to beta-actin and expressed as % untreated macrophages.