Supplemental figure 1: Protocols for isolated adult ventricular rat cardiomyocytes without/with hypoxic preconditioning (HPC). ACh: acetylcholine; AChR: acetylcholine receptor; H/R: hypoxia/reoxygenation; TC: time control; reoxy.: reoxygenation.Supplemental Figure S2: Preliminary and control experiments. Atropine concentrations higher than 0.1 µmol/L (A) and Hexamethonium in concentrations higher than 1 mmol/L (B) interfere with cardiomyocyte viability after H/R. The incubation with the acetylcholine receptor antagonists atropine (0.1 µmol/L) and hexamethonium (1 µmol/L) had no impact on cardiomyocyte viability in controls with H/R or TC per se (C). Data are presented as means±standard deviations. Cardiomyocytes were isolated from n=8 hearts (4 males, 4 females, marked in blue or red). The atropine concentration finally used in experiments is underlayed in gray. The hexamethonium concentration finally used in experiments is underlayed in gray. H/R: hypoxia/reoxygenation, TC: time control.Supplemental figure S3: Neuronal marker protein class III β-tubulin is present in tissue lysates of rat atria (A) and ventricles (B), but not in the isolated adult ventricular rat cardiomyocyte (C). Left: Correlation between relative fluorescence signal intensity of TUBB3 (green, right axis) or GAPDH (red, left axis) and the protein concentration from pooled samples (n=12, respectively). Right: The membrane sections, used for signal intensity analysis. Marker proteins are visualized via red and green fluorescence signals, respectively. The star symbol indicates the reference sample (pooled lysate from atria, ventricles and isolated adult ventricular rat cardiomyocytes), which was loaded on each gel. Full uncut membranes are displayed in the Supplemental Fig. S4. GAPDH: glyceraldehyde 3-phosphate dehydrogenase, TUBB3: class III β-tubulin.Supplemental Figure S4: Supplementary Western Blot data. Increasing protein concentrations from pooled rat atrial, ventricular, and cardiomyocyte samples, respectively (A, B): Full unedited ...