Additional file 1: Figure S1. Isolation of rice meiocytes and unicellular microspores and quality control of rice male germ cells BS-seq. Figure S2. DNA methylation levels in reproduction cells compared with somatic tissues. Figure S3. Analysis of microspore hyper DMRs relative to meiocyte and sperm. Figure S4. Analysis of mCHG genes in unicellular microspore. Figure S5. Effects of cmt3a and cmt3b in pollen development. Figure S6. CMT3b maintains high mCHG level in microspore. Figure S7. Analysis had no effect on mCHG in cmt3a mutant sperm cells. Figure S8. Production and phenotypic analysis of jmj706/707 double mutants. Figure S9. Average methylation level of TEG and genes in jmj706/707 meiocyte and sperm cells compared with wild type. Figure S10. Effect of the cmt3a/b and jmj706/7 mutations on sexual lineage-specific methylation. Fig. S11. Analyze the Canonical SLH and lineage-specific methylation (SLM). Figure S12. Effects of cmt3a and cmt3b mutations in zygote and/ or egg DNA methylation. Figure S13. Transcriptome data analysis. Figure S14. Enrichment of upregulated genes in cmt3b meiocyte and sperm. Figure S15. DNA methylation levels in rice reproductive cells and seedlings. Figure S16. DNA CHH methylation landscape in rice reproductive cells and seedlings.