Additional file 1: Fig. S1. Signaling by Gαi1/q chimeras. (A) HEK293 cells were transfected with 0.4 μg/mL of various Gαi1/t1 constructs. Cells were labeled with myo-[3H]inositol and then assayed for [3H]IP accumulation. Data shown are mean ± SEM of one triplicate experiment. (B) Expression of Gαi1 or Gαq constructs were confirmed by immunoblotting with 20 μg of total protein.