Copyright information:Taken from "IL-12 Production Induced by Fraction H (ABH) Involves Toll-like Receptor (TLR)"Evidence-based Complementary and Alternative Medicine 2004;1(3):259-267.Published online Jan 2004PMCID:PMC538514.© 2004, the authors . The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. ABH (0.17 ng/ml) and reference LPS (10 ng/ml) were pre-treated with 5 μg of polymyxin B for 30 min, and then used to stimulate PBMC for 16 h. The IL-12 secreted into the supernatant was measured by ELISA.