Copyright information:Taken from "The unusually large telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus"Nucleic Acids Research 2005;33(3):1111-1122.Published online 18 Feb 2005PMCID:PMC549419.© The Author 2005. Published by Oxford University Press. All rights reserved () Diagram indicating the source of two different antibodies used to characterize PfTERT: a mouse serum raised against a GST–PfTERT fusion protein and a rabbit serum raised against a 15 amino acid peptide at the PfTERT C-terminus (C-PfTERT peptide). () The 48 h time course in blood stages, during which parasites develop inside an erythrocyte. PfTERT (green) was detected by IF using the rabbit anti-peptide antibody; nuclear DNA was stained with DAPI (blue). () Peptide competition assay. The specificity of PfTERT serum was determined by IF after pre-incubating the antibodies with increasing concentrations of a specific (C-PfTERT) or an unspecific peptide. Detection of anti-PfTERT antibodies and staining of DNA was performed as in (B).