Copyright information:Taken from "Complex interactions of HIV-1 nucleocapsid protein with oligonucleotides"Nucleic Acids Research 2006;34(2):472-484.Published online 24 Jan 2006PMCID:PMC1351370.© The Author 2006. Published by Oxford University Press. All rights reserved () Binding of NC to (TG). One min injections of (from bottom to top) 0, 1, 2.5, 5, 10, 25, 50, 100 and 200 nM NC were passed over a surface of 344.2 RU's (TG) at 64 µl/min. () Rate of binding of NC to (TG) as a function of NC concentration. Initial slopes in (A) were measured using BIAevaluation 3.01, and the plot of initial slopes versus NC was fit using linear regression. () Effect of (TG) in solution on binding of NC to immobilized (TG). A total of 100 nM NC was incubated at 4°C with (from top to bottom) 0, 0.1, 0.25, 0.5, 1.0, 2.5, 5.0, 10.0, 25.0, 50.0 and 100.0 µM (TG) for at least 1 h before injecting a sample for 1 min at 64 µl/min.