Copyright information:Taken from "Formation of functional Tat translocases from heterologous components"BMC Microbiology 2006;6():64-64.Published online 19 Jul 2006PMCID:PMC1550398. A. Strains MC4100 (parental strain; ; WT) and JARV16-P (as MC4100, Δ/Δ, ; Δ/) were grown aerobically to late exponential phase in LB medium prior to harvesting and fractionation into membrane (M) and soluble (C) fractions. Samples were separated by SDS PAGE, electroblotted and probed with anti TatA antiserum. B. Strain JARV16-P (Δ/) or JARV16-P carrying pUniprom-SA encoding TatA (ScoA) were cultured, fractionated and blotted as above, and probed with anti TatA peptide antiserum. C. Strain M15 [pREP4] harboring either pQEAQ1 (AaeA1) or pQEAQ2 (AaeA2) encoding TatA1 or TatA2 with C-terminal histags, respectively. Cells were cultured in LB medium until OD600 of 0.4 was reached, after which expression of the TatA protein was induced by addition of 1 mM isopropyl-β-D-galactopyranoside (IPTG) for a further 2 hours. Cells were fractionated and blotted as above, and probed with anti pentahis antiserum. For each panel, membrane and soluble material from an equivalent amount of cells was loaded.