Copyright information:Taken from "tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells"Nucleic Acids Research 2005;33(6):1902-1912.Published online 4 Apr 2005PMCID:PMC1074395.© The Author 2005. Published by Oxford University Press. All rights reserved () Effect of the attachment of tRNA on the kinetics of hybridization between different molecular beacons and full-length β-actin mRNA. The green curves show the results obtained from the real-time monitoring of hybridization reactions performed with molecular beacons that are not linked to tRNA, and red curves show the results obtained from the real-time monitoring of hybridization reactions containing the same molecular beacons linked to tRNA. () Imaging the distribution of β-actin mRNA in chicken embryonic fibroblasts using Texas red-labeled molecular beacons linked to tRNA and TMR-labeled free molecular beacons. The cells were imaged with respect to each fluorophore 1 h after cytoplasmic injection of the mixture of probes. () Merged images of cells that remained mobile. The red color represents the fluorescence of the tRNA-linked molecular beacons and the green color represents the fluorescence of molecular beacons not linked to tRNA.