Copyright information:Taken from "Genetic transformation of novel isolates of chicken bearing probiotic features for expression of heterologous proteins: a tool to develop live oral vaccines"BMC Biotechnology 2006;6():2-2.Published online 5 Jan 2006PMCID:PMC1360068.Copyright © 2006 Mota et al; licensee BioMed Central Ltd.striction fragments onto pCR2.1-TOPO vector. The genes AM and mut2 were also PCR amplified from other plasmids and used as selective marker and reporter gene, respectively.