Copyright information:Taken from "Characterization of the SECIS binding protein 2 complex required for the co-translational insertion of selenocysteine in mammals"Nucleic Acids Research 2005;33(16):5172-5180.Published online 9 Sep 2005PMCID:PMC1214547.© The Author 2005. Published by Oxford University Press. All rights reserved Samples of 5 mg of crude extracts containing untagged CTSBP2 were resolved by Sephadex 200 gel filtration chromatography in the presence and absence of 1 M NaCl. Fractions were analyzed by western analysis using affinity-purified anti-SBP2 antibody. The position of peak fractions relative to standards is shown by an arrow along with calculated molecular weight. The elution time for XH-CTSBP2 is shown for reference.