Copyright information:Taken from "Characterization of the SECIS binding protein 2 complex required for the co-translational insertion of selenocysteine in mammals"Nucleic Acids Research 2005;33(16):5172-5180.Published online 9 Sep 2005PMCID:PMC1214547.© The Author 2005. Published by Oxford University Press. All rights reserved () A sample of 1.25 mg of cytoplasmic extracts (14 000 supernatants [S14]) from McArdle 7777 cells stably expressing V5/His-tagged FLSBP2 (VH-FLSBP2) were resolved by Sephacryl S-500 gel filtration chromatography in either low salt (150 mM NaCl) or high salt (1 M NaCl) () or as indicated. Fractions were analyzed by western analysis using an anti-V5 antibody. () Purified XH-FLSBP2 (30 pmol) was chromatographed under the same conditions in the presence of 2 nmol purified salt-washed rat ribosomes. () A sample of 300 pmol of XH-FLSBP2 was chromatographed in the absence of added ribosomes. The fractions for (C) and (D) were analyzed by western analysis using an anti-SBP2 antibody. The calculated peak molecular weights are shown at the bottom.