Copyright information:Taken from "Induction of Proinflammatory Cytokines and C-Reactive Protein in Human Macrophage Cell Line U937 Exposed to Air Pollution Particulates"Environmental Health Perspectives 2005;113(11):1536-1541.Published online 21 Jul 2005PMCID:PMC1310915.This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI. () The levels of CRP in whole-cell lysates from U937 macrophages 48 hr after treatment with 10 μg/cm DEP, UDP, sDEP, sUDP, OE-DEP, OE-UDP, or 1% PBS as vehicle control (C) were determined by Western blot analysis. Equivalent amounts of whole-cell lysates (100 μg protein) were loaded in each lane on 10% SDS-polyacrylamide gels and analyzed by immunoblotting using a CRP- or actin-(housekeeping protein as control) specific antibody. () Densitometric evaluation of CRP protein band intensities normalized to actin protein band intensities. Mean values of two independent experiments are shown.