Copyright information:Taken from "Evaluation of reference-based two-color methods for measurement of gene expression ratios using spotted cDNA microarrays"BMC Genomics 2006;7():35-35.Published online 24 Feb 2006PMCID:PMC1402275.Copyright © 2006 Peixoto et al; licensee BioMed Central Ltd. Breast comparison from all technical replicates is shown. The yellow dots display the logintensity ratio (M) as a function of the logintensity product (A) for each gene on the array. Spots that were excluded from further analyses (with intensities below the local background plus 2 SD) are shown in gray. Green lines show the credibility interval thresholds (see Methods) used for estimation of differentially expressed genes.