Copyright information:Taken from "Evaluation of reference-based two-color methods for measurement of gene expression ratios using spotted cDNA microarrays"BMC Genomics 2006;7():35-35.Published online 24 Feb 2006PMCID:PMC1402275.Copyright © 2006 Peixoto et al; licensee BioMed Central Ltd. Breast comparisons are shown. Upper panels: scatter plots between direct ratios and each of the three reconstructed ratios (RefOligo, RefPool, One-Color). Lower panels: scatter plots between the reconstructed ratios. Ratio plots for the remaining tissue comparisons are shown as supplementary material [see Additional file 2]. In all cases, only ratios from spots with intensity values 2 SD above the average slide background were used.