Ctively. The expression of CD80, CD83, CD86 and HLA-DR was analyzed on fixed cells by FACScalibur flow cytometer and data analysis was carried out with FlowJo software. The PBMCs were gated for the CD14 positivity. The results of a representative experiment are shown; the shadowed curve represents the untreated cells.Copyright information:Taken from "Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation"http://www.biomedcentral.com/1471-2105/9/S2/S5BMC Bioinformatics 2008;9(Suppl 2):S5-S5.Published online 26 Mar 2008PMCID:PMC2323668.