Copyright information:Taken from "Kinetics of double-chain reversals bridging contiguous quartets in tetramolecular quadruplexes"Nucleic Acids Research 2006;34(8):2386-2397.Published online 8 May 2006PMCID:PMC1458523.© The Author 2006. Published by Oxford University Press. All rights reserved () Experimental data at two wavelengths (240 and 295 nm) and fitted functions (solid lines). oligonucleotide at 60 µM strand concentration in a 0.11 M Na, pH 7.2 buffer at 3°C. The fitted values were 1.62 × 10 and 1.81 × 10 M.s at 240 and 295 nm, respectively. () Effect of strand concentration (10–80 µM) for at 3°C in 0.11 M Na on the relative absorbance at 295 nm. Quadruplex formation leads to an increase in absorbance at this wavelength. () Effect of temperature on association for at 3°C (blue), 8°C (orange) and 12°C (red) in 0.11 M Na on the absorbance at 240 nm. Quadruplex formation leads to a decrease in absorbance at this wavelength. () Arrhenius plots for the association of (circles), (triangles), (squares), (closed squares) and TGT (squares; dotted line) oligonucleotides. () Effect of pH on association. is plotted versus pH for , d(GCGGGGAT, closed circles) and dTGT (red symbols). Solid and dotted lines, exponential fits (note the -axis log scale) for the data points. () Effect of ionic strength on association. is plotted versus [Na] for , d(GCGGGGAT, closed circles) and dTGT (red symbols). These experiments were performed in a 10 mM lithium cacodylate pH 7.2 buffer with various concentrations of NaCl.