Protein molecular weights are indicated on the right. (B and C) Images of hippocampal neurons infected with LV/EGFP-Synapsin1a or LV/EGFP-Synapsin1a/Bsn16 and immunostained with antibodies against MAP2 (blue), Bassoon (B, red), or Piccolo (C, red). Bars, 10 μm. (D and E) Bar graphs quantifying the percent colocalization of Bassoon (D) or Piccolo (E) immunoreactive puncta with EGFP-Synapsin1a clusters along dendritic profiles. (F) Dispersion and reclustering kinetics of EGFP-Synapsin1a in control boutons (EGFPSyn) and those lacking Piccolo (EGFPSynPclo28) or Bassoon (EGFPSynBsn16). EGFPSyn and EGFPSynPclo28 curves are from (n = ∼100 puncta per condition). Error bars indicate SEM. (G) Destaining kinetics of FM5-95 during 5-Hz stimulation for control boutons (EGFPSyn) and those lacking Piccolo (EGFPSynPclo28) or Bassoon (EGFPSynBsn16). > 200 puncta, two experiments per condition. Error bars indicate SEM.Copyright information:Taken from "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis"The Journal of Cell Biology 2008;181(5):831-846.Published online 2 Jun 2008PMCID:PMC2396795.