The broken gray line indicates maximal amount of dispersion (expressed as a percentage of initial fluorescence intensity before stimulation) seen in these neurons without KN62 ( > 100 puncta/condition). (B) Dispersion kinetics of Synapsin1a in neurons lacking Piccolo and incubated with (KN62) or without (control) KN62 during a 90-s, 10-Hz stim. For comparison, the broken gray line indicating maximal amount of dispersion observed in wild-type neurons is included ( > 100 puncta per condition). (C) Bar graphs summarizing the percent dispersion of EGFP-Synapsin1a puncta at synapses with (EGFPSyn) or without (EGFPSynPclo28) Piccolo in the presence or absence of KN62. Percent dispersion (100 − the percentage initial fluorescence) was measured at 90 s after a 10-Hz, 90-s stimulation ( = 2 experiments, 905 puncta for control without KN62; three experiments, 1,070 puncta for control + KN62; seven experiments, 2,195 puncta for Pclo28 without KN62; eight experiments, 2,341 puncta for Pclo28 + KN62). (D) Destaining kinetics of FM5-95 at boutons lacking Piccolo in the presence (triangles) or absence (squares) of KN62, during a 5-Hz, 180-s (900 pulse) stimulation ( = 5 experiments, 1,134 puncta for Pclo28 without KN62; two experiments, 437 puncta for Pclo28 + KN62). Error bars indicate SEM.Copyright information:Taken from "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis"The Journal of Cell Biology 2008;181(5):831-846.Published online 2 Jun 2008PMCID:PMC2396795.