. Each sample comprised 25 μl of milk whey and was run under reducing conditions. (a) Representative analysis of S100A12 expression in milk whey from cow 1419. Lane 1, control quarter pre-infection; lane 2, control quarter post-infection; lane 3, pre-infection control from the quarter receiving 1 × 10; lane 4, post-infection sample (1 × 10bacteria); lane 5, pre-infection control from the quarter receiving 1 × 10; lane 6, post-infection sample (1 × 10bacteria). Arrows denote the positions of S100A12 immunoreactive oligomers (monomer, dimer, trimer) detected using the immunoaffinity purified antibodies raised to rS100A12. (b) Analysis of PTX3 expression in milk whey. Lane 1, cow 1419 pre-infection sample from the quarter receiving 1 × 10; lane 2, post-infection sample (1 × 10bacteria); lane 3, cow 1419 pre-infection sample from the quarter receiving 1 × 10; lane 4, cow 1419 post-infection sample (1 × 10bacteria); lane 5, cow 1490 pre-infection sample from the quarter receiving 1 × 10; lane 6, cow 1490 post-infection sample (1 × 10bacteria); lane 7, cow 1592 pre-infection control from the quarter receiving 1 × 10; lane 8, cow 1592 post-infection sample (1 × 10bacteria). Arrows denote the positions of PTX3 immunoreactive bands (monomer and dimer) detected using immunoaffinity purified antibodies raised to rPTX3.Copyright information:Taken from "Identification of immune genes and proteins involved in the response of bovine mammary tissue to infection"http://www.biomedcentral.com/1746-6148/4/18BMC Veterinary Research 2008;4():18-18.Published online 2 Jun 2008PMCID:PMC2430192.