Copyright information:Taken from "Common determinants in DNA melting and helicase-catalysed DNA unwinding by papillomavirus replication protein E1"Nucleic Acids Research 2006;34(10):3008-3019.Published online 31 May 2006PMCID:PMC1474052.© 2006 The Author(s) () Footprints of E1 with and without ATP and PS1βH mutants as indicated above the lanes. Lanes 1–3, reactions without ATP; free probe, 25 and 50 nM wild-type E1. All other reactions contained ATP/Mg (5 mM). Lanes 4 and 23, reactivity of free probe. Lanes 4–7, wild-type E1, 10–50 nM. Lanes 8–10, R505E, 25–125 nM. Lanes 11–13, K506E, 75–300 nM. Lanes 14–16, H507A, 20–80 nM. Lanes 17–19, H507L, 25–100 nM. Lanes 20–22, H507F, 15–60 nM. G is a Maxam–Gilbert DMS marker ladder. () Footprints of hydrophobic loop mutants as indicated above the lanes. Lanes 1 and 20, reactivity of free probe. Lanes 2–4, wild-type E1, 10–50 nM. Lanes 5–7, F464A, 25–125 nM. Lanes 8–10, F464L, 25–125 nM. Lanes 11–13, F464W, 25–100 nM. Lanes 14–16, W465A, 50–200 nM. Lanes 17–19, L466A, 30–125 nM.