A 15-day interval, b) a single dose of liposomes (25 μg) by intranasal instillation, c) four doses of naked DNA (100 μg each) by intramuscular route at a 15-day interval or d) one dose of BCG (Moreau strain) given by subcutaneous injection of about 10live bacteria in 100 μl saline. Thirty days after the last dose, mice were challenged intra-tracheally with H37RV and 30 days post infection we performed CFU analysis. Data represent the mean logCFU counts ± SD of six mice per group of one of three independent experiments. *p < 0.01 were considered significant when compared to the saline group.Copyright information:Taken from "Protection against tuberculosis by a single intranasal administration of DNA-hsp65 vaccine complexed with cationic liposomes"http://www.biomedcentral.com/1471-2172/9/38BMC Immunology 2008;9():38-38.Published online 22 Jul 2008PMCID:PMC2500095.