Istry for the H1aV fusion-protein on representative coronal sections approximately −1 and +1 mm from the injection site, 8 weeks after bilateral injection of rAAV1/2-H1aV/-empty. (–, left panels) Z-projections of the GFP-enhanced H1aV expressing green cells/neurites (oriens layer, stratum radiatum, and molecular layer) on NeuN positive Cy-3 fluorescent neurons reveal transgene expression almost exclusively in the hippocampus. rAAV-empty injected brains show no GFP fluorescence antibody enhancement. (–, middle panels) DAB stainings of adjacent sections demonstrating robust labeling of pyramidal cell bodies in all subregions of the dorsal hippocampus. (, middle panel) No DAB positive cells were detected in hippocampi of rAAV-empty injected mice. (–, right panels) High-magnification confocal dual fluorescent images (H1aV in green, NeuN in red) of representative regions from the CA1 pyramidal cell layer (marked by white boxes in – left panels) showing ∼80% double labeling over the total extent of the dorsal hippocampus. Note that the extent of transgene expression in the hippocampus of rAAV-infected cells is comparable with the distribution of H1aV in TgH1aV.Fb mice (Figure C inset). (, right panel) No GFP fluorescence was detected in rAAV-empty infected neurons. αNeuN, anti-NeuN staining; αGFP, anti-GFP staining.Copyright information:Taken from "Select Overexpression of Homer1a in Dorsal Hippocampus Impairs Spatial Working Memory"Frontiers in Neuroscience 2007;1(1):97-110.Published online Jan 2007PMCID:PMC2518050.