Alternative dsRNA delivery using solid food. Groups of 100 3th instar-larvae from Ae. aegypti RJ strain were fed with three plugs of 300 μl each containing 300 μg of VGSC422 dsRNA complexed with PEI and dissolved in 2 % of pre-melted agarose and food solution. Then, larvae were reared until adult stage and exposed to 0.5 μg/ml of deltamethrin, as described in A. The percentage of mortality after 30 min is shown. Statistics: Two-way Anova, followed by Sidak’s multiple comparison test. *P