The Florida Fish and Wildlife Conservation Commission’s Florida Marine Research Institute (FWC-FMRI) has examined total mercury levels in muscle tissue from a variety of economically and ecologically important species as part of an ongoing study to better understand mercury contamination in marine fishes. The FWC-FMRI Mercury Program is one of the most comprehensive programs in the United States for monitoring mercury levels in marine and estuarine fishes. Because mercury, a toxic metallic element, has been shown to bioaccumulate in fish tissue, humans consuming fish can potentially consume significant levels of mercury. We examined the concentration of total mercury in 6,806 fish, representing 108 species from 40 families. Species represented all major trophic groups, from primary consumers to apex predators. The majority of individuals we examined contained low concentrations of mercury, but concentrations in individual fish varied greatly within and among species. Species with very low mean or median mercury concentrations tended to be planktivores, detritivores, species that feed on invertebrates, or species that feed on invertebrates and small fish prey. Apex predators typically had the highest mercury concentrations. In most species, mercury concentration increased as fish size increased. Sampling in Florida waters is continuing, and future research relating mercury levels to fish age, feeding ecology, and the trophic structure of Florida’s marine and estuarine ecosystems will help us better understand concentrations of this element in marine fishes. Study areas: Choctawhatchee Bay, Apalachicola Bay, Cedar Key, Tampa Bay, Sarasota Bay, Charlotte Harbor, Florida Bay, Florida Keys, Tequesta, southern Indian River Lagoon, Indian River Lagoon, Volusia County, and northeast Florida.